The laboratory is mainly used for teaching laboratory methods for molecular biology. There is a processing of DNA molecules consisting of isolation of plant and animal DNA, followed by DNA quality and quantity control, amplification of DNA regions of interest and restriction digestion evaluated by electrophoretic separation. When working with proteins, students will try their isolation and separation using 2D electrophoresis. Furthermore, the laboratory is used for research activities in the field of quantification of gene expression using qPCR and nanopore sequencing.
Main activities
- Teaching basic methods in molecular biology
- Work with DNA - isolation, amplification by PCR, restriction digestion
- Work with proteins - isolation, separation, 2D SDS PAGE
- Nanopore DNA sequencing
- Realization of bachelor and diploma theses in the field of molecular biology
Laboratory equipment
- Real-Time PCR system croBEE
- Isoelectric focuser PROTEAN i12 IEF
- DNA / RNA UV-decontamination box UVC / T-AR Biosan
- NanoPhotometer N60 Touch nanospectrophotometer
- OSMOMAT 3000b cryoscopic eightometer
- Nikon E TS100-F microscope
- Cecil CE3041 spectrophotometer
- METTLER analytical balances
- Horizontal electrophoretic system Thermo scientific OWL easycast B1A, Minigel system
- Vertical electrophoretic system Mini-PROTEAN Tetra Vertical Electrophoresis Cell
Responsible person
